How much protein can a western blot detect?
1 Answer
Nov 13, 2015
depends on various factors. I have detected 2
Explanation:
there are various factors which are involved in the process which include.
- amount of protein in cell lysate
- effectiveness/sensitivity of the antibodies
- substrate sensitivity used for chemiluminescence
i have detected about 2
with purified protein i could detect approximate 1
but above three factors are crucial.