The dilution factor will be 500, or 1:500.
Basically, you start with the volume of steel-acid solution you have in the #"100-mL"# flask. For a solution, the dilution factor is defined as the ratio between the final volume - after the dilution - and the initial volume - before the dilution
#"DF" = V_"final"/V_"initial"#
Your initial volume before the dilution will be #"1 mL"#, the aliquot you take from the solution you've prepared. After you dilute the aliquot, the final volume will be #"500 mL"#. As a result,
#"DF" = V_"final"/V_"initial" = "500 mL"/"1 mL" = "1:500"#
This is the equivalent of adding #"499 mL"# to your #"1-mL"# aliquot for a dilution factor of 1/500.
Read more about dilution factors here:
http://socratic.org/questions/how-do-you-calculate-dilution-factor?source=search
http://socratic.org/questions/how-do-you-do-serial-dilution-calculations?source=search