Question #ab798
1 Answer
Anion exchange chromatography aims at separating and retaining anions, or negatively charged particles, by using a positively charged absorbent functional group.
The same principle I described for cation exchange chromatography applies here, i.e. like charges repel and opposite charges attract.
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In the case of anion exchange chromatography, the positively charged stationary phase particles will separate and retain the negatively charged proteins.
Addendum
Here's a nice image I found showing the anion-exchange chromatography process
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Notice that the anions bind to the positively charged static phase particles; at the same time, the positively charged proteins go through without being retained.
