Question #c18bc

1 Answer
Ernest Z.
Jul 31, 2017

The concentrations in the 10X running buffer are "A) [Tris] = 0.250 mol/L"; "B) [Gly] = 1.92 mol/L"; "C) [SDS] = 1 % (m/v)"A) [Tris] = 0.250 mol/L;B) [Gly] = 1.92 mol/L;C) [SDS] = 1 % (m/v).

Explanation:

A) Tris

"Moles of Tris" = 6.06 color(red)(cancel(color(black)("g Tris"))) × "1 mol Tris"/(121.14 color(red)(cancel(color(black)("g Tris")))) = "0.050 02 mol"

"[Tris]" = "0.050 02 mol"/"0.200 L" = "0.250 mol/L"

B) Glycine

"Moles of Gly" = 28.8 color(red)(cancel(color(black)("g Gly"))) × "1 mol Gly"/(75.1 color(red)(cancel(color(black)("g Gly")))) = "0.3835 mol"

"[Tris]" = "0.3835 mol"/"0.200 L" = "1.92 mol/L"

C) SDS

"[SDS]" = "2 g"/"200 mL" × 100 % = "1 % (m/v)"

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